getHybridizationCandidatesFrame(...)
Signature: primerSequenceMap:(type) -> frameForHybridisation:(type) -> (type)
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Returns a frame with the blast results with the lowest evalue for all primer candidates. This corresponds to the first match with no 100% identity
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getResultFrameWithHybridisationEnergies(...)
Signature: bcContext:(type) -> hybridizationCandidatesFrame:(type) -> (type)
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returns a frame containing the selfalign, internal loop, and cross hybridization energy for the input primer candidates using IntaRNA for prediction.
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readCleanedBlastResultFrame(path)
Signature: path:string -> (type)
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reads a cleaned (no comment lines) blast result and filters identical matches
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sortFrameForHybridization(...)
Signature: cleanBlastResultFrame:(type) -> (type)
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removes unnecessary columns from the blast result frame and groups primers by query id, generation index, direction (fwd/rev) and row index
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